Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons <em>In vivo</em>
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چکیده
منابع مشابه
Retrograde Fluorescent Labeling Allows for Targeted Extracellular Single-unit Recording from Identified Neurons In vivo
The overall goal of this method is to record single-unit responses from an identified population of neurons. In vivo electrophysiological recordings from individual neurons are critical for understanding how neural circuits function under natural conditions. Traditionally, these recordings have been performed 'blind', meaning the identity of the recorded cell is unknown at the start of the reco...
متن کاملExtracellular Single-Unit Recording Methods*
Since their refinement in the early 195Os, extracellular, singleunit recording methods have been used to obtain a wealth of data about the properties of CNS structures. The applications of the technique have been diverse: extracellular microelectrodes have been used to map the potential fields of single discharging neurons in order to answer fundamental questions about the excitability of CNS d...
متن کاملPrinciples of Extracellular Single-Unit Recording
8 There is a rather large gap between membrane properties of individual neurons and behavior or physiological events. To bridge this gap, we need to be able to understand the activity of individual neurons and how that activity contributes to neuronal circuits. Extracellular microelectrode recording, which focuses on neuronal action potentials of individual neurons as the “code” used by the int...
متن کاملAn evaluation of photoengraved microelectrodes for extracellular single-unit recording.
Microelectrodes fabricated using integrated-circuit technology are shown to be suitable for extracellular single-unit recording, and the factors influencing electrode-cell coupling are discussed. Electrode tip size as well as location is found to be critical in recording high spike amplitudes, suggesting that the paths for current flow in central nervous system (CNS) tissue may be extremely small.
متن کاملA guide to in vivo single-unit recording from optogenetically identified cortical inhibitory interneurons.
A major challenge in neurophysiology has been to characterize the response properties and function of the numerous inhibitory cell types in the cerebral cortex. We here share our strategy for obtaining stable, well-isolated single-unit recordings from identified inhibitory interneurons in the anesthetized mouse cortex using a method developed by Lima and colleagues. Recordings are performed in ...
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ژورنال
عنوان ژورنال: Journal of Visualized Experiments
سال: 2013
ISSN: 1940-087X
DOI: 10.3791/3921